Chinese alias
Hydrogen chloride ethanol solution 30-35%
English alias
hydrochloric acid in ethanol
HYDROGEN CHLORIDE 1.25M IN ETHANOL
hydrogen chloride - ethanol solution
HYDROGEN CHLORIDE ~1.25 M IN ETHANOL, 250 ML
Belonging category
Inorganic chemical industry: inorganic acids
Density 0.825 g/mL at 20 ° C
Flash point 21 ° C
Storage conditions 2-8 ° C
PH value 1.0 (H2O, 20 ° C)
Warning word danger
Dangerous goods markings F, T
Hazard category code 11-23-35
Safety instructions 26-36/37/39-45
Dangerous goods transport number UN 2920 8/PG 1
WGK Germany1
F3
Customs code 3822 00 0
biological activity
Hydrochloric acid ethanol solution is a differentiation solution commonly used in routine pathological hematoxylin eosin (HE) staining. The commonly used solution is obtained by dissolving 0.5%~1% hydrochloric acid in 70% ethanol solution. It is precisely because it is so common that relevant pathology and technical books rarely introduce its mechanism of action, especially the role of ethanol.
Experimental principle
Under acidic conditions, it can effectively wash away hematoxylin staining outside the nucleus, thereby reducing background; At the same time, it is also possible to weaken the staining in areas where the nucleus is too dark, making the staining clearer.
application
The components of hydrochloric acid ethanol differentiation solution have been repeatedly optimized, and the differentiation speed is very fast, usually only about 2-5 seconds, which can be completed very quickly. It is particularly suitable for experimental personnel who are very proficient in hematoxylin staining differentiation operation. After staining with hematoxylin, the tissue slices were treated with differentiation medium, and the nuclear cytoplasmic staining became clearer and more distinct.